BIOTAQ Unprecedented Single-Cell Affymetrix GeneChip Service for Only $650!
From RNA to Data For Only $650 Per Sample!
Flat Fee for Both Small Samples (with 10pg of total RNA) & Standard Samples (with 1ug of total RNA)!
Laser Capture Microdissection (LCM) makes it possible to obtain small cell populations, ideally, microarray gene expression analysis should be performed from homogeneous cell populations, it is therefore required sometimes to do gene expression from a single cell. BIOTAQ now provides expert Affymetrix gene expression profiling service from a single cell.
With our optimized protocol and proprietary procedures for linear RNA amplification, we offer you high quality from_RNA_to_data GeneChip service, we do both small & standard sample amplification (as small as 10 pg of RNA from single cell), FLAT FEE of $650 for both small & standard sample amplification. We only need you to send us as little as 10 pg of total RNA, we will deliver you the high quality microarray data for your analysis. With your money, we will get your samples thru the following steps:
- First strand & second strand cDNA synthesis from your RNA sample, followed by clean-up of double-stranded cDNA.
- First round cRNA synthesis to generate cold cRNA, followed by clean-up of cRNA.
- Second round cDNA synthesis, followed by clean-up of cDNA.
- Second round cRNA synthesis to generate biotinylated cRNA, followed by cRNA clean-up and its quality will be checked by Agilent 2100 to determine its length.
- cRNA is fragmented by using optimized experimental conditions and its length of fragmented cRNA will be checked by Agilent 2100.
- Fragmented cRNA is mixed with hybridization mix, and will be hybridized to the array of your choice.
- Array will be washed and scanned & raw expression profile data will be collected and analyzed.
- CD including raw data and Excel formatted data will be mailed to you together with array images, scatter plots, cRNA QC data, array QC data.
Turnaround time for standard RNA samples (with more than 1ug of total RNA) will be 8 business days.
Turnaround time for small RNA samples (with less than 200 pg of total RNA) will be 15 business days.
Interested in our Affymetrix GeneChip(R) Services?
Please contact us via email: sales@biotaq.com or you can simply fill out the feedback form at:http://www.biotaq.com/Feedback.htm
We will contact you shortly after we receive it. Thanks.
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Phase Separation Tube for RNA Isolation (phase separation buffer in it, 15ml scale), 4 Preps (Cat: BT100-009D), $30
Phase Separation Tube (phase separation buffer in it) & binding buffer for spin column, 4 Preps (Cat: BT100-009B), $25
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