Sensitivity-enhanced RNA LabChip® Sampling Buffer(SN-RLSB) was developed
to be used on Agilent 2100 Bioanalyser, to provide the enhanced
sensitivity for RNA 6000 Nano Kit, it can achieve 40 to 150-fold
increase in sensitivity depending on the nature of
your samples, it allows you to boost the detection limit of RNA 6000 Nano Kit for total RNA
down to 20pg/µl, even lower than RNA 6000 Pico Kit’s
detection limit (200pg/µl). Most importantly, all you need to do is to
just replace the RNA 6000 Nano Marker with this
Sampling
Buffer, detection of total RNA down to 20pg
on Nano chip can be easily achieved.
Fig. 1 (300ng
w/o SN-RLSB) Fig. 2 (75ng w/ SN-RLSB)
 
Agilent Traces of CEgrade RNA Ladder (Low Range) with RNA 6000 Nano
Marker (Fig.1, RNA Loading: 0.3ug)
and
Sensitivity-enhanced Sampling Buffer (Fig. 2, RNA
Loading: 75ng; Fig. 3 RNA Loading: 1.5ng). Peaks
refer to 1.0, 0.8, 0.6, 0.4, 0.3,
0.2, 0.1
kb
respectively (from left to right).
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