INSTCoating Solution for EOF Elimination in CE

Biotaq’s proprietary INSTCoating solution provides instant coating to reduce or eliminate electroosmotic flow (EOF) in capillary electrophoresis applications. When rinsed through the capillary, the novel, pre-made polymer in the solution is adsorbed onto the inner wall of silica tubing, forming a highly uniformed, stationary coating layer through dynamic interactions.

The INSTCoating solution provides similar performance to linear polyacrylamide coating. The polyacrylamide coating is an irreversible chemical process. After using it for a while, the capillary needs to be disposed. Unlike linear polyacrylamide coating, the INSTCoating process is reversible and can be rinsed off and be recoated again for many times. This results in significant cost savings without requiring the purchase of new polyacrylamide coated capillary every time.

Features and Benefits

  • Useful for applications where you need to suppress EOF and reduce analyte-wall interactions in capillary electrophoresis
  • Significant cost savings over polyacrylamide coated capillary
  • Reversible and can be rinsed off and recoated again for many times
  • 20 to 30 injections are possible for one coating
  • No absorbance above 250 nm
  • Stable between 20°C and 50°C
  • The whole coating procedure is fast and simple (see operating protocol)
  • To learn more, see product sheet

Quotes from Our Customers

"I have used INSTCoating solution for several years. It is an excellent product."

Dr. Petr Tůma, Associate Professor, Institute of Biochemistry
Cell and Molecular Biology, Charles University in Prague

Examples of CE Separation Using INSTCoating Solution

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Fig. 1 Determination of amino acids in human plasma

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Fig. 2 Determination of inorganic and organic anions in cell medium

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Fig. 3 Determination of neurotransmitters in brain tissues


Reference

Tůma P, Sustková-Fišerová M, Opekar F, Pavlíček V, Málková K. (2013). Large-volume sample stacking for in vivo monitoring of trace levels of γ-aminobutyric acid, glycine and glutamate in microdialysates of periaqueductal gray matter by capillary electrophoresis with contactless conductivity detection. Journal of Chromatography A, 1303, 94–99

Tůma P, Opekar F, Samcová E, Stulík K. (2013). The use of a multichannel capillary for electrophoretic separations of mixtures of clinically important substances with contactless conductivity and UV photometric detection. Electrophoresis, 34, 2058–2064

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Specifications and Prices
Catalog Number Volume Price
BT200-01
2 ml
$285

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