INSTCoating Solution Operating Protocol

  1. INSTCoating solution is intended for use on new bare fused silica capillary that has never been treated or coated with other chemicals before.
  2. Prior to coating, place 0.1M NaOH, DD water, INSTCoating solution and running buffer (the buffer that you use for your applications) into four different reservoirs.
  3. Pressurize the reservoir that contains 0.1M NaOH to push it into the fused silica tubing and flush it for 5 minutes.
  4. Pressurize the reservoir that contains DD water to push it into the fused silica tubing and flush it for 2 minutes.
  5. Pressurize the reservoir that contains INSTCoating solution to push it into the fused silica tubing and flush it for 2 minutes.
  6. Pressurize the reservoir that contains DD water to push it into the fused silica tubing and flush it for 3 minutes.
  7. Pressurize the reservoir that contains running buffer to push it into the fused silica tubing and flush it for 4 minutes.
  8. After the coating procedure is done, you can load your separation polymer and inject the sample into the capillary to proceed to the electrophoresis separation step.